- Systems Biology
- Cell Biology
- Logical Modelling
I am a master student in biotechnology at NTNU officially specialising in molecular biology, but with a particular interest in systems biology to solve challenges within my field. Through my studies I have gained experience in computational and experimental work, and have a desire to utilise both these skills for my master thesis.
Title: Computational simulations and experimental validation of mode-of-action of novel compounds affecting macrophage-mediated adverse effects.
In this project, a monocyte-derived macrophage logical model developed previously by other Master students, will be tested to see whether it can represent the regulatory mechanisms of Kupffer cells. If this proves to be the case, further tests of systematic perturbations of the model to predict Kupffer cell biomarker patterns will be performed. Obtained RNAseq data from either human blood-derived macrophages and in vitro grown Kupffer cells will be used to train the model and to compare model stable states with published observations. The macrophage model will be upgraded to optimally represent Kupffer cell important receptors and pathways. Based on an initial analysis of the dry lab observations, the most interesting predictions will be tested through wet lab experiments. This project will be carried out in collaboration with Coegin Pharma – Follicum.
Martin Kuiper – IBI, Berit Johansen – IBI, Eirini Tsirvouli, IBI